KMID : 1007520140230062045
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Food Science and Biotechnology 2014 Volume.23 No. 6 p.2045 ~ p.2052
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Antioxidant Activities of Novel Small-molecule Polysaccharide Fractions Purified from Portulaca oleracea L.
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Li Yu-Ping
Yao Li-Hua Wu Guan-Jie Pi Xiao-Fang Gong Yan-Chun Ye Ruo-Shong Wang Chen-Xi
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Abstract
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Novel small-molecule polysaccharide fractions, named POP II and POP III, were purified from Portulaca oleracea L. with average Mw values of 9.25 and 8.03 kDa, respectively. Monosaccharide analysis revealed that POP II was composed of Rha, Ara, Man, Glc, and Gal with molar ratios of 1: 1.42: 0.44: 0.88: 1.59. POP III was composed of Rha, Ara, Glc, and Gal with molar ratios of 1: 1.16: 0.23: 0.59. The antioxidant activities of the fractions were evaluated using cell-free and cell-mediated radical generating systems. POPII and POP III possessed strong antioxidant activities in both systems. The 2 novel polysaccharide fractions extracted from P. oleracea L. can be developed as natural antioxidants for treatment of free radical-related diseases.
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KEYWORD
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Portulaca oleracea L., polysaccharide, antioxidant activity, hydroxyl radical, reactive oxygen specie
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